Do you centrifuge lavender top tubes?
Centrifuge for the preprogrammed time or 10 minutes for red or gold top tubes, 10 minutes for green, and 15 minutes for BD Blue top tubes. If minimum g-force (1300g) cannot be achieved, spin for 20 minutes. Do not use a manual brake on the centrifuge. Only un-spun tubes may be placed in pneumatic tube…
Centrifuge for the preprogrammed time or 10 minutes for red or gold top tubes, 10 minutes for green, and 15 minutes for BD Blue top tubes. If minimum g-force (1300g) cannot be achieved, spin for 20 minutes. Do not use a manual brake on the centrifuge. Only un-spun tubes may be placed in pneumatic tube system.Click to see full answer. Considering this, what is a lavender top tube used for?Purple or lavender: K2 EDTA. This is a strong anticoagulant and these tubes are usually used for complete blood counts (CBC). Lavender top tubes are generally used when whole blood is needed for analysis. Can also be used for some blood bank procedures such as blood type and screen.Beside above, do you centrifuge gray top tubes? Sodium fluoride tubes (gray top): Gently mix specimen by inverting 5-6 times and place immediately at 4°C for up to 30 minutes before spinning in a centrifuge. If blood is placed into tubes after removing the tube tops, care must be taken not to cross contaminate tubes containing anticoagulant. Accordingly, how many times do you invert a lavender tube? Immediately after collection, invert the tube 8-10 times to mix anticoagulant. Green NHP – 6 ml. Sodium Heparin Special tests requiring heparinized plasma or whole blood Immediately after collection, invert the tube 8-10 times to mix anticoagulant. Lavender LAV – 4 ml.What are the three elements in an anticoagulated tube once centrifuged?Briefly, blood is collected in dry glass tubes or glass-coated plastic tubes and immediately softly centrifuged. Three layers are formed: a red blood cell (RBC) base at the bottom, acellular plasma (platelet-poor plasma (PPP)) as a supernatant, and a PRF clot in the middle (Fig. 1).
