How do you make a 5% cell suspension?
Place 0.2 -0.5 ml of blood into the tube (2-3 drops). Fill the tube with the saline. Centrifuge at 200 G for 1-2 minutes until the RBC’s are packed. Decant the supernatant. Tap the tube to resuspend the RBC’s in the residual fluid. To make a 5% cell suspension add 1 volume of the packed…
Place 0.2 -0.5 ml of blood into the tube (2-3 drops). Fill the tube with the saline. Centrifuge at 200 G for 1-2 minutes until the RBC’s are packed. Decant the supernatant. Tap the tube to resuspend the RBC’s in the residual fluid. To make a 5% cell suspension add 1 volume of the packed RBC’s to 19 volumes of saline. Click to see full answer. Thereof, how do you make a 5% suspension? Place 0.2 -0.5 ml of blood into the tube (2-3 drops). Fill the tube with the saline. Centrifuge at 200 G for 1-2 minutes until the RBC’s are packed. Decant the supernatant. Tap the tube to resuspend the RBC’s in the residual fluid. To make a 5% cell suspension add 1 volume of the packed RBC’s to 19 volumes of saline. how do you make a red blood cell suspension? Preparation of Pooled Cell Suspension: Place 1 drop of red cells each from 3 of O group sample tubes or segment into the O labelled tube. Fill the tube ¾ full with 0.9% saline to resuspend the cells. Centrifuge the tubes for at least 2 to 3 minutes on high speed. Decant the supernatant fluid. In this regard, why do you need to prepare red cell suspension at 2 5 %? Preparation of a 2-5% cell suspension provides cells in an optimum concentration to detect weak antibodies. Wash bottle with isotonic saline.What is red cell suspension?A red cell suspension is a common reagent used for many serologic procedures. Red cell suspensions provide the appropriate serum to cell ratio to allow for grading and interpretation of tests results.
