What does kcat stand for?
Kcat, or k2 or turnover number (they all mean the same thing) is a measure of how many substrates one (1) enzyme can convert into a product per second.Click to see full answer. In this manner, what kcat means?kcat is the turnover number, the number of times each enzyme site converts substrate to product per…
Kcat, or k2 or turnover number (they all mean the same thing) is a measure of how many substrates one (1) enzyme can convert into a product per second.Click to see full answer. In this manner, what kcat means?kcat is the turnover number, the number of times each enzyme site converts substrate to product per unit time. This is expressed in the inverse of the time units of the Y axis. It is the substrate concentration needed to achieve a half-maximum enzyme velocity.Similarly, is kcat the same as Vmax? Kcat is equal to Vmax/[Enzyme]. Because the concentration of enzyme is taken into account in this equation, Kcat does NOT vary with the amount of enzyme used and is therefore a constant for an enzyme. Kcat is equal to the number of molecules of product made per enzyme per unit time. One may also ask, what does kcat mean in enzyme kinetics? Kcat is the turnover number — the number of substrate molecule each enzyme site converts to product per unit time. If you know the concentration of enzyme sites, you can fit Kcat instead of Vmax when analyzing a substrate vs. velocity curve.How do you calculate kcat?Note: the enzyme concentration is the same for all of the test tubes; only the substrate concentrations vary in the assay. Divide the Vmax (from Section 2, Step 4) by the enzyme concentration (from Section 2, Step 5). The result is the value of Kcat.
