What is nuclease free water?
Nuclease free water is used in order to dilute the concentration of the reagents to the proper final concentration. Also use of nuclease free water helps avoid DNA degradation by nucleases as well as interference of the PCR reaction by ions which could be present in otherwise not nuclease free deionized water.Click to see full…
Nuclease free water is used in order to dilute the concentration of the reagents to the proper final concentration. Also use of nuclease free water helps avoid DNA degradation by nucleases as well as interference of the PCR reaction by ions which could be present in otherwise not nuclease free deionized water.Click to see full answer. Then, what is the purpose of nuclease free water?Nuclease-free Water is ideal for the preparation of reagents and for use in enzymatic reactions. No toxic agents, such as DEPC, are used in the production of this water, so as to avoid inhibition in enzymatic reactions.One may also ask, what does nuclease free mean? Nuclease free water means free from both nuclease. DNase is normally not very stable. It can be deactivated by vortexing or heating but RNase are more stable. In case of RT you either use RNase free water or nuclease free water. Beside this, how do you make nuclease free water? Popular Answers (1) Add 0.1% DEPC to MilliQ or Double Distilled water – let it sit overnight at 37degC and then Autoclave it. Make sure the glassware used is also rinsed with the same water or treated with Chloroform or Baked in a hot air oven (260degC) for 4hrs. It should be ready for use – both DNase and RNase free.Is MilliQ water nuclease free?In practice, milliQ water suffices for most standard PCR (you don’t need to buy nuclease free water or DEPC-treat your water). If you want to exercise an abundance of caution, use nuclease free water. While DNA can be stored in water, unbuffered water is not great for long-term stability.
