What is thioglycollate broth used for?
Thioglycolate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. Sodium thioglycolate in the medium consumes oxygen and permits the growth of obligate anaerobes.Click to see full answer. Considering this, how do you make thioglycollate broth? Preparation and Method of Use of Thioglycollate Broth Suspend 29g in 1…
Thioglycolate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. Sodium thioglycolate in the medium consumes oxygen and permits the growth of obligate anaerobes.Click to see full answer. Considering this, how do you make thioglycollate broth? Preparation and Method of Use of Thioglycollate Broth Suspend 29g in 1 liter of distilled water. Bring to the boil and dissolve the medium completely. Distribute into tubes or bottles and sterilize by autoclaving at 121°C for 15 minutes. Inoculate the medium following aseptic technique. Secondly, why it is important not to agitate the thioglycollate broth? When evaluating tubes of FTM (Fluid Thioglycollate Media) it is important not to agitate the tubes because the position of growth in the medium can be easily changed if handled carelessly. Similarly, what does fluid thioglycollate medium reduce oxygen? Sodium thioglycollate is a reducing agent which removes molecular oxygen from the medium and prevents the accumulation of peroxides which may be lethal to some microorganisms. Sulfhydryl groups inactivate mercury and other heavy metals neutralizing the antibacterial effect of mercurial preservatives.What is the function of sodium thioglycollate in the FTM?A feature of sodium thioglycollate, in addition to lowering the oxidation-reduction potential, is its ability to neutralize the antibacterial activity of mercurial compounds. These characteristics make FTM particularly useful for determining the presence of contamination in biological and other materials.
