How do you prepare red blood cell suspension?

Popular Answers (1) 1 drop of blood were put in the centrifuge tube. Added saline in it until there is 1cm left from the tube mouth. Then centrifuge it at 2500-3000 rpm for about 1-2 minutes. After centrifuge the supernatant are removed and blood are mixed well with another saline. The step 2-3 are repeated. Click to see full answer. In this regard, how do you make a red blood cell suspension? Preparation of Pooled Cell Suspension: Place 1 drop of red cells each from 3 of O group sample tubes or segment into the O labelled tube. Fill the tube ¾ full with 0.9% saline to resuspend the cells. Centrifuge the tubes for at least 2 to 3 minutes on high speed. Decant the supernatant fluid.Similarly, how do you clean red blood cells? Step 1: Centrifuge the whole blood at 3000rpm (1800rcf) for 5 minutes Step 2: Remove plasma and buffy coat layer. Step 3: Resuspend the red cells in normal saline (0.9% NaCl) with approximately 2 times the volume of the red cells, and invert the tube to mix. Likewise, people ask, what is the purpose of preparing a red cell suspension in the laboratory? 1.0 Principle A red cell suspension is a common reagent used for many serologic procedures. Red cell suspensions provide the appropriate serum to cell ratio to allow for grading and interpretation of tests results.Why wash red blood cells with saline?Saline Washed Red Blood Cells. Saline washed RBCs must be used within 24 h after washing since the original collection bag has been entered, which breaks the hermetic seal and increases the possibility of bacterial contamination. Removal of the anticoagulant-preservative solution also limits cell viability and function